<EM>D</EM>-半乳糖-1-<sup>13</sup>C的合成与表征

D-半乳糖-1-¹³C的合成与表征

摘要: 以硝基甲烷¹³C为原料合成D-半乳糖-1-¹³C。采用分离中间体硝基糖醇的方式，简便拆分差向异构体D-半乳糖和D-塔罗糖，合成产物的总产率为42.6%，¹³C丰度为99.1%，光学纯度为100%，化学纯度为99.7%。以高效液相色谱蒸发散射光检测器（HPLC-ELSD）、液相色谱-质谱联用仪（LC-MS）和核磁共振（¹H NMR）对所得产物进行结构表征，确定产物结构为D-半乳糖-1-¹³C。

Abstract: D-Galactose-1-¹³C was synthesized with nitromethane ¹³C as starting material. The separation was performed of the epimers of nitro D-Galactitol and nitro D-talitol, which resulted in D-Galactose being obtained and the resolution of the epimers of D-Galactose and D-taltose being avoidance. D-Galactose-1-¹³C was characterized by HPLC-ELSD, LC-MS and ¹H NMR. The fine structure and ¹³C abundance of it were done by LC-MS and ¹H NMR. The yield of ¹³C-labeled D-Galactose was 42.6% with 99.1% ¹³C abundance, 100.0% optical purity and 99.7% chemical purity.