Abstract: Using n-octane as processing gas and single centrifuge as separator, a series of experiments were conducted to study the separation performance. Samples of each experiment were sent to mass spectrometer for analysis, and the overall separation factor and flow cut at different feed flow and holdup were obtained. The overall spearation factor were all close to or more than 1.10, and the maximum valum can reach to 1.13. Based on the experimental data, parameters of cascade for separating ¹³C were estimated using the MARC model. The calcuation results indicated that ¹³C can be neriched to about 11% by centrifuge cascade.