13C6-1,2:5,6-O-双异丙叉-α-D-呋喃葡萄糖的合成

¹³C₆-1,2:5,6-O-双异丙叉-α-D-呋喃葡萄糖的合成

摘要: 采用浓硫酸为催化剂，以D-葡萄糖-¹³C₆和丙酮为原料合成了¹³C₆-1,2:5,6-O-双异丙叉-α-D-呋喃葡萄糖，并对其进行了气相色谱（GC）、质谱（MS）分析。对合成条件进行了优化，优化后的合成条件为：葡萄糖与丙酮的摩尔比为1∶73.5，浓硫酸用量为10 mL，反应温度25 ℃，反应时间12 h，优化条件下合成的¹³C₆-1,2:5,6-O-双异丙叉-α-D-呋喃葡萄糖收率为75.6%，产品中¹³C丰度为99.2 %，纯度为98.7%。

Abstract: With concentrated sulfuric acid as the catalytic agent, ¹³C₆-1,2:5,6-Di-O-isopropylidene-α-D-glucofuranose was synthesized with D-glucose-¹³C₆ and acetone. ¹³C labeled 1,2:5,6-Di-O-isopropylidene-α-D-glucofuranose was characterized by GC and MS. The reaction conditions were optimized to be as follows: the molar ratio of D-glucose-¹³C₆∶acetone = 1∶73.5, the volume of concentrated sulfuric acid = 10 mL, the reaction temperature = 25 ℃, and the reaction time = 12 h. Under these conditions, the yield of ¹³C₆-1,2:5,6-Di-O-isopropylidene-α-D-glucofuranose was 75.6% (based on D-glucose-¹³C₆), with 99.2% ¹³C atom abundance and 98.7% chemical purity.