血清3,5,3’-三碘甲腺原氨酸酶联免疫分析试剂盒的研制

Establishment of an Enzyme Linked Immunosorbent Assay for Triiodothyronine

摘要: 以辣根过氧化物酶标记链亲合素(SA)，T₃抗体包被微孔，T₃生物素化，四甲基联苯胺(TMB)为底物，建立了3,5,3’-三碘甲腺原氨酸（T₃）生物素-亲合素酶联免疫分析(T₃-BA-EL1SA)方法。结果显示，本方法分析灵敏度为0.2 μg/L，批内、批间变异系数分别为7.1％～8.3％和6.5％～10.5％，回收率为98.1％～107.2％；高浓度T₃血清样品系列倍比稀释后，测定值与稀释度呈线性相关，相关系数为0.995 7。本试剂盒操作简便、快速，适用于临床检测和科研应用。

Abstract: A sensitive and specific ELISA for Triiodothyronine was established by using anti-T₃ antibody coated on the microtiter plate，T₃ biotinlated．The labeling was horseradish peroxidase(HRP)conjugate of streptavidin．TMB-H₂O₂ solution was used as the substrate of HRP．The sensitivity of the assay was 0.2 μg/L．The intra- and inter-assay CVs were 7.1％-8.3％ and 6.5％-10.5％ respectively．The analytical recovery was 98.1％-107.2％, the coefficient correlation was 0.995 7. This method for measuring T₃ is rapid，sensitive and convenient．It is suitable for clinical and research application．