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Octreotide的131I标记及Graves 眼病炎症细胞的体外摄取

Labeling of Octreotide With 131I and Its Vitro Uptake in Inflammatory Cells for Graves’ Ophthalmopathy

    摘要
  • 摘要: 将octreotide中的苯丙氨酸用酪氨酸替换合成Tyr3octreotide，对其进行了131I标记。考察了氯胺T标记法中氯胺T和Tyr3octreotide的用量对标记率的影响，观察了Graves眼病相关炎症细胞对131I-Tyr3octrotide 的体外摄取情况。结果表明，对octreotide进行结构修饰后得到的Tyr3octreotide可以用于131I标记；氯胺T法对Tyr3octreotide进行131I标记时，氯胺T和Tyr3octreotide的最佳用量分别为1.6和1.4 μg，最高标记率为85%；T淋巴细胞、活化的成纤维细胞及血管内皮细胞均能够摄取131I-Tyr3octreotide，而脂肪细胞的摄取则明显较少。以上结果表明，131I-Tyr3octreotide能够被Graves眼病发病过程中的炎症细胞摄取，具有用于Graves眼病发病过程中炎症反应程度评估的应用潜力。但由于摄取量还很小，需要进一步深入研究。

     

    Abstract: Phenylalanine was displaced by tyrosine in octreotide(Tyr3octreotide) to study the labeling methods of Tyr3octreotide with 131I，and the difference uptake ability of 131I-Tyr3octreotide in various cell lines of Graves ophthalmopathy were observed. The results showed that Tyr3octreotide could be labeled with 131I by chloramines-T method and the optimal conditiona was 1.6 μg chloramines-T and 1.4 μg Tyr3octreotide. The labeling efficiency was 85%. T lymphocyte, fibroblast, and HUVEC could accumulate 131I-Tyr3octreotide largely compared to lipocyte, which indicated that 131I-Tyr3octreotide could be combined with inflammatory cells in Graves’ ophthalmopathy.

     

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