<sup>13</sup>C<SUB>3</SUB>-三硬脂酸甘油酯的合成与表征

¹³C₃-三硬脂酸甘油酯的合成与表征

摘要: 以¹³C₃-甘油和硬脂酸为原料，在无溶剂的条件下，采用Lipozyme酶为催化剂，直接酯化合成¹³C₃-三硬脂酸甘油酯，产率为80%，产物无需纯化。经差示扫描量热分析（DSC）、红外光谱（FT-IR）、核磁共振（H NMR）、质谱（MS）分析表征，结果表明，产物为目标产物¹³C₃-三硬脂酸甘油酯，化学纯度＞98%，同位素丰度＞99%，产物可作为同位素内标物使用。

Abstract: A highly efficient synthesis of ¹³C₃ labeled triglycerides of stearic acids from ¹³C₃-glycerol and stearic acids, by immobilized lipase-catalyzed in solvent-free medium was described. The structure of the product were characterized by fourier transform infrared spectrum (FT-IR), nuclear magnetic resonance (NMR), mass spectra (MS). The results showed that triglycerides of stearic acids contained three ¹³C atoms. The isotope abundance of ¹³C₃-tristearin was more than 99% and the yield was 80% of ¹³C₃-tristearin through calculation. Chemical purity (>98%) was obtained by differential scanning calorimetry (DSC).