自制美沙西汀-甲氧基-<sup>13</sup>C的表征

自制美沙西汀-甲氧基-¹³C的表征

摘要: 采用新的方法，以甲醇-¹³C为原料直接合成了美沙西汀-甲氧基-¹³C，并对其进行了HPLC、LC-MS和¹HMNR等分析。结果表明，所合成的样品是目标产品，产率为70.0%，¹³C丰度为99%，纯度为99.8%。与经典的合成方法比较，本方法具有较好的经济效益。使用本方法自制的美沙西汀-甲氧基-¹³C进行小鼠¹³C-呼吸试验，结果表明，美沙西汀-甲氧基-¹³C可以在小鼠活体状态下正确评估其损伤和恢复情况。

Abstract: Methacetin-methoxy-¹³C was synthesized by using methanol-¹³C with a novel method, and the characterization of it was performed using HPLC, LC-MS and ¹HMNR. The results indicated that the synthetic was right. And the yield of methacetin-methoxy-¹³C was 70.0% with 99% ¹³C abundance and 99.8% purity. Compared with the classical method， there was more benefit. The methacetin ¹³C-breath test was performed with the synthetic on the live mice, which showed a precise reflection of alteration of liver function in liver injury and functional recovery.