Abstract: An applicable deuterium analysis method of deuterium depleted water was experimentally studied by used H₂ carrier and the chromatogram columniation with 5A molecular sieve at natural temperature after decompounding the deuterium depleted water by magnesium at 500 ℃. Decompounding the water using the magnesium instead of uranium can avoid the radioactive danger and the isotope effect was smaller than zinc. Determination by chromatograph at natural temperature can overcome the operation complexity at liquid nitrogen and the difficulty of determining the emend factor of the H₂, HD, D₂. The results showed that standard curved using standard sample of super low heavy water can reversely extend to below the natural deuterium abundance，deuterium content of deuterium depleted water can be calculated. The relative standard deviation of the method＜5%， recovery rate with added specimen with three addition level was 96.6%-99.9%， the combined relative uncertainty was 0.166. It can meet the need of quantitative analysis during the deuterium depleted water manufacturing.