Abstract:
3H11 was labeled with
123I by Iodogen method, and the labeling product were purified with PD-10 column. The labeling yield and the radiochemical purity of the product was determined by paper chromatography. The biodistribution of
123I-3H11 in normal mice was carride out as well. The optimal experimental conditions of
123I-3H11 was as follow: Iodogen 10 μg, 3H11 30 μg, Na
123I solution 20 μL（13.3 MBq）, PBS 100 μL（pH 7.4，0.2 mol/L）, the normal temperature for 8 min. The labeling yield of
123I-3H11 was 70%80%. After stored at 4 ℃ for 48 h in human serum, the radiochemical purity was more than 92%. The results of biodistribution showed that the clearance of radiolabeled antibody in blood (half time, T
1/2) was 12.25±0.25 h, and the radioactivity in the stomach was uptaken obviously. The above results indicated that
123I-3H11 appears to show some potential as gastric cancer imaging diagnostic agent.
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