Abstract: ¹⁷⁷Lu was produced by thermal neutron bombardment on natural Lu₂O₃ target in the research reactor and obtained as ¹⁷⁷LuCl₃ solution after corresponding chemical process. EDTMP kits containing Ca with different formulations were prepared, and ¹⁷⁷Lu labelling of these kits was evaluated under different conditions such as pH, reaction time, reaction volume and the molar ratio of Lu to EDTMP. The biodistribution and imaging experiments were performed in normal male SD rats. The results of labelling indicated that the labelling yields could be obtained over 95%, and the stability of the reaction solution was also satisfactory. The results of biodistribution and imaging demonstrated that ¹⁷⁷Lu-EDTMP showed significant uptake and strong retention in bones and it was excreted mainly via urinary system and cleared very rapidly from blood and soft tissues. The images showed no noticeable accumulation of ¹⁷⁷Lu-EDTMP in main organs and soft tissues except in bladder. ¹⁷⁷Lu-EDTMP prepared by kit method exhibited desired in vivo distribution properties as a potential therapeutic radiopharmaceutical for bone pain palliation.