O-(2-18F氟乙基)-5-羟基-L-色氨酸(5-18FEHTP)的合成动物体内分布及MicroPET显像

Synthesis, biodistribution of animal and MicroPET Imaging of O-(2-18Ffluoroethyl)-5-hydroxy-L-try- ptophan (5-18FEHTP)

摘要: 通过对现有CTI公司计算机控制的化学合成模块(CPCU) 进行改造，首次合成了L-5-羟基色氨酸类似物(5-18FEHTP)，并用高效液相(HPLC) 检测其放化纯度，所得的产品用于昆明小鼠的S180肉瘤模型显像。结果显示，采用改进方法合成5-18FEHTP的总时间是45min，纯化时间是20min，放化收率为12-16%(n=15)，产品的放化纯度大于98%。MicroPET显像结果表明，5-18FEHTP在S180肉瘤浓集程度明显高于周围其它组织。以上结果提示利用CPCU半自动合成5-18FEHTP，方法简便、稳定、产品纯度较高。动物生物分布和显像结果表明5-18FEHTP可能成为一种新的PET显像剂。

Abstract: 5-18FEHTP was synthesized by using a modified chemistry process control unit (CPCU). Radiochemical purity was over 98% measured by High Performance Liquid Chromatograph (HPLC). After injecting of 5-18FEHTP through tail vein, the mice was scanned with Micro PET. The no decay corrected radiochemical yield of 5-18FEHTP was 12-16% (n=15) within 45min total reaction time, and the manually purified time was about 20min. 5-18FEHTP can be conveniently synthesized with modified CPCU and the labeling yield was stable, the results of Micro PET imaging indicated that 5-18FEHTP may be a new promising PET tracer.