Abstract: 2-18F-A-85380 was synthesized by the modified chemistry process control unit (CPCU). The radiochemical purity and specific radioactivity were measured by High Performance Liquid Chromatograph (HPLC). After tail vein injection of 2-18F-A-85380, the rat was scanned with MicroPET and the imaging result was compared with that of 18F-FDG. The decay-corrected radiolabeled yield of 2-18F-A-85380 was 50±5 % (n=6) within 45 min total reaction time. The radiochemical purity was around 99％ according to the analytical HPLC. The specific radioactivity was 3.78±0.54 Ci/µmol. 2-18F-A-85380 was successful in visualizing thalamus, which is rich of Nicotinic acetylcholine receptor (nAChRs) compared with other tissues. There was no significant uptake of 18F-FDG in thalamus. 2-18F-A-85380 could be conveniently synthesized with modified CPCU and the labeling yield was stable, the MicroPET imaging result indicates that 2-18F-A-85380 could be suitable for clinical translation.