流加对前体物发酵法合成15NL色氨酸的影响

Effect of Fed-batch on Synthesis of 15N L-tryptophan from Precursor Fermentation

摘要: 以经过诱变和5-甲基色氨酸处理的假丝酵母突变株AS60为出发菌株，研究了在前体物发酵合成15NL-色氨酸过程中，分别流加葡萄糖、15N硫酸铵及15N邻氨基苯甲酸对发酵的影响。结果表明：在发酵36h后，分别流加1.5 g/L 的15N邻氨基的苯甲酸、2.1 g/L的15N硫酸铵以及50 g/L葡萄糖时，可使发酵液中15NL-色氨酸的产量达到3.073g/L。

Abstract: Using Candida utilis AS60 as 15N L-tryptophan producing strain，the influence by different feeding modes of glucose, 15N (NH4)2SO4 and 15N anthranilic acid was studies in the paper. The results of these experiments show the yield of 15N L-tryptophan was 3.073g/L by addition of 50 g/L of glucose, 2.1 g/L of 15N (NH4)2SO4 and 1.5 g/L of 15N anthranilic acid after 36 h of fermentation.