磺胺甲恶唑酶联免疫分析方法的建立

The Establishment of an Competition Inhibition (ci) ELISA for sulfamethoxazole

摘要: 本方法建立了磺胺甲恶唑酶联免疫分析方法用以检测动物源性食品中磺胺甲恶唑的残留。利用辣根过氧化物酶（HRP）标记SMX（Sulfamethoxazole ），兔抗SMX抗体制备固相抗体，通过标本中的SMX和一定酶标SMX竞争结合固相抗SMX多抗，标本中SMX的量和显色后的OD₄₅₀值呈负相关的原理来检测标本中的SMX含量。经方法学鉴定，本方法的灵敏度为0.067μg/L，批内变异<10%，批间变异<20%。牛奶、肉类、鸡蛋、蜂蜜样品的回收率分别为94.9%~116.0%、 82.0%~107.6%、 81.6%~94.9%、89.0%~93.0%，符合免疫分析方法的要求，

Abstract: A sulfamethoxazole-specific ELISA was developed to analyze sulfamethoxazole The preparation of horseradish peroxidase labeled sulfamethoxazole（SMX）and polyclonal antibody coated plates are described.The principle for the measurement of SMX is that the SMX in the sample and the enzyme-labeled SMX competitively is bound to the polyclonal antibody against the SMX in the solid phase, And the signal falls with the increasing SMX concentration. The sensitivity of the assay is 0.067μg/L.The intra-and intro-assay CVs are less than 10% and 20% respectively. The analytical recoveries rate of milk, meat, egg and honey are in the range of 94.9%~116.0%,82.0%~107.6%,73.0%~90.0%and61.9%~94.0%respectively,A sulfamethoxazole-specific ELISA is established to analyze SMX residues in animal-based food products.