高效液相色谱法测定尿素<sup>13</sup>C混合粉中的尿素<sup>13</sup>C含量

高效液相色谱法测定尿素¹³C混合粉中的尿素¹³C含量

摘要: 用高效液相色谱法测定了尿素13C混合粉中的尿素13C含量。用Alltech Econosphere NH2柱（250mm×4.6mm，5µm）作色谱柱，色谱条件为：V(乙腈):V(甲醇):V(水)=900：100：10为流动相，流速为1mL•min-1，检测波长为200nm，进样量为20 µL。尿素13C在0.2～1.0 mg•mL-1浓度范围内，呈良好的线性关系，回归方程为：y＝2.548×106x＋4.005×104 ，r=0.9999，平均回收率为100.6％。本法简便、准确，适于尿素13C混合粉的质量控制。

Abstract: A HPLC method is developed for determination of Urea 13C in Urea 13C Mixed Powder. A Alltech Econosphere NH2 column (250 mm×4.6 mm,5μm)is used as stationary phase, a mixture of V(acetonitrile):V(methol):v(water)=900:100:10is used as mobile phase and the flow rate is 1 mL•min-1, UV detection wavelength is performed at 200 nm. The calibration curve shows good linearity in the range of 0.2-1.0 mL•min-1 of Urea 13C, y＝2.548×106x＋4.005×104 ，r=0.999 9, and the averaged recovery is 100.6%. The method is simple and accurate, and can be used for the quality control of Urea 13C Mixed Powder.