125I示踪法研究rhPTH(1～34)在大鼠体内的药代动力学
Study on the Pharmacokinetics of rhPTH1-34 in Sprague-Dawley
Rats by 125I Tracing
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摘要: 采用125I标记重组人甲状旁腺素（1～34）肽（Recombinant Human Parathyroid hormone（1～34），rhPTH（1～34））示踪技术，研究rhPTH(1～34)在SD大鼠体内的药代动力学。结果显示，SD大鼠单次皮下给予1.8和3.6μg/kg剂量的rhPTH1-34后，其主要药代动力学参数为：达峰时间Tmax为0.26±0.07 h和0.25±0.0 h，血浆峰浓度为1.30±0.17 μg/L和3.20±1.12μg/L，末端消除半衰期T1/2el为1.70±0.56 h和1.58±0.70 h，药物浓度－时间曲线下面积AUC(0-∞)为3.1±0.7 μg•h /L和6.7±0.7 μg•h /L。表明大鼠单次皮下给予rhPTH(1～34)后，在给药后大约0.25 h血药浓度达到峰值，随后出现一个较快的消除过程，其药代动力学特征符合非静脉给药线性一房室模型一级动力学。Abstract: 125I tracing method is used to observe the pharmacokinetic parameters of the recombinant human parathyroid hormone (1-34) (rhPTH1-34) in adults Sprague-Dawley rats. The results show that when adults SD rats is given a dosing regimens of 1.8 and 3.6μg/kg rhPTH1-34 by single dose subcutaneous, the main pharmacokinetic parameters of rhPTH1-34 including Tmax, Cmax, T1/2el and AUC is 0.26±0.07h, 0.25±0.0h; 1.30±0.17 μg/L, 3.20±1.12μg/L; 1.70±0.56h, 1.58±0.70h and 3.1±0.7 μg•h /L,6.7±0.7μg•h /L, respectively. It shows that the pharmacokinetic characteristics of rhPTH1-34 in SD rats is accord with linear one-compartment model and eliminate with mono-phase kinetics.
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