Abstract: Quantitative protein expression profiling is a crucial part of proteomics and requires technique that are able to efficiently provide accurate,high-throughput and reproducible differential expression values for proteins in two or more biological samples.At present,stable isotope labeling is probably considered as one of the most accurate ways to relatively quantify protein expression levels and additionally stable isotope labeling may be directly combined to LC MS/MS approaches.In summary,this technique has its advantages in quantitative proteomics.The application and the latest progresses about this technique are discussed.