氯霉素酶联免疫分析方法的建立

Establishment of an Enzyme Linked Immunoassay for Chloramphenicol

摘要: 以兔抗氯霉素多抗为包被抗体,氯霉素(CAP)辣根过氧化物酶连接物为标记物,四甲基联苯胺(TMB)为显色底物,建立了氯霉素直接竞争酶联免疫分析(CAP-EIA)方法。本方法灵敏度为0.046μg/L,批内变异系数<10%,批间变异系数<24%,回收率62%~145%。用牛奶样品预处理所得上清液为稀释液做得稀释实验,相关方程为y=3.988x-0.234,相关系数为0.999。标准曲线范围为0.1~10μg/L。

Abstract: A sensitive and specific EIA for chloramphenicol(CAP) is established by using one anti-CAP polyclonal antibody.The antibody is coated on the microtiter plate.The labelled compound is horseradish peroxidase(HRP) conjugate of chloramphenicol.TMB-H_2O_2 solution is used as the substrate of HRP.The sensitivity of the assay is 0.046 μg/L.The intra-and inter-CVs of 10 samples are lower than 10% and 24%,respectively.The analytical recovery is 62%～145%.The correlative equation is y=3.988x-0.234,r=0.999,using milk supernatant as buffer.The analytical range is 0.1～10 μg/L.