Abstract: 100 dyspeptic patients are performed an endoscopy with biopsy for histology, rapid urease test,13C-urea breath test(13C-UBT) and ASSURE?Hp rapid Test(HpRT). Patients are considered to be infected if both histology and biopsies yield positive results, and not infected when both tests are negative. The pathology and persons responsible for endoscopy, urease test and 13C-UBT are unaware of the results from the other diagnostic methods. The 13C-UBT has the following results: sensitivity 96%.specificity 95%; rapid urease test has the following results: sensitivity 79%, specificity 76%; HpRT has the following results; sensitivity 86%.specificity 88%. 13C-UBT provides excellent sensitivity and specificity for the diagnosis of Hp. Pylori infected Hp, it can be the first choice for detecting the infection of the Hp.