多种~(117)Sn~m(~(113)Sn)-配体体系的形成条件及其与BSA的结合

Investigation on Formation Conditions and Combination With BSA of Various ~(117m,113)Sn-Iigand Systems

摘要: 以117Snm(113Sn)示踪法研究了Sn—DTPMP、Sn—DTPA、Sn—EDTMP、Sn—HEDT、MP、Sn—TTHMP体系的形成条件,同时研究了不同标记体系与牛血清白蛋白(BSA)的结合性质。研究表明,除DTPA外,其他配体在较高浓度下均能与Sn4+形成络合,但配体浓度较低时锡极易水解,用生理盐水稀释和增加体系pH均加剧水解。研究同时显示,水解锡标记体系与BSA有很强的结合,未水解锡标记体系与BSA的结合率远低于水解锡标记体系。

Abstract: Formation conditions of 117m,113Sn-DTPMP,117m,113Sn-DTPA,117,113Sn-EDTMP,, 117m,113Sn-DTPMP systems are investigated. The results show that Sn4+ is very vulnerable to hydrolysis, when the concentration of ligand is low, and the hydrolysis is accelerated by dilution with saline or increased with pH value especially. Combination of various systems with bovine serum albumin (BSA) is also studied. The results indicate that all kinds of SnO2·H2O·yligand combine strongly with BSA, while the combination percentages of Sn4+-ligands with BSA are much lower than those of SnO2·xH2O·yligands.