洛伐他汀对血管平滑肌细胞AP-1结合活性的影响

The Effect of Lovastation on AP-1 Binding Activity in Vascular Smooth Muscle Cells

摘要: 采用~(32)P标记寡核甘酸探针,用电泳迁移率变动分析法(Electrophoretic Mobility Shift Assay,EMSA)检测转录因子AP-1结合活性,以此观察洛伐他汀对血管平滑肌细胞AP-1结合活性的影响。结果显示,洛伐他汀能明显降低血管平滑肌细胞AP-1结合活性(降低72.6％±5.2％)。

Abstract: Nuclear factor AP-1 binding activity is detected by electrophoretic mobility shift assay (EMSA), and the effect of Lovastatio on AP-1 binding activity in vascular smooth muscle cells is investigated by using 32P labled Oligonucleotide. The results show that Lovastatin strongly inhibits AP-1 binding activity reduced 72. 6%±5. 2%.