时间分辨荧光免疫分析和放射免疫分析测量精度的比较

The Comparison Between the Measurement Precision of RIA and TRFIA

摘要: 采用时间分辨荧光免疫分析和放射免疫分析法对血清样品进行分析,对其加样精度、测定精度和系统精度进行了比较。结果表明:①血清加样时,手动加样CV>3.4％,自动加样CV1.3％,自动加样CV<0.3％,P<0.001。②测量精度,放免法CV=2.4％,时间分辨法CV=0.8％,P<0.001。③系统精度,放免法CV=3.6％,时间分辨法CV=1.3％,P<0.001。以上结果提示:时间分辨荧光免疫分析在加样精度、测定精度和系统精度上都显著优于放射免疫分析。

Abstract: The measurement precision of sampling, counting system and the systemic precision between RIA and TRFIA are compared with randomized patient sample testing. The precision of sampling has significant difference between manual sampling (CV> 3. 4%) of RIA and auto sampling (CV<0. 7%) of TRFIA in serum sampling (P<0. 001). The precision of sampling has significant difference between manual sampling (CV>1. 3%) of RIA and auto sampling (CV<0. 3%) of TRFIA in reagent dispensing (P<0. 001). The precision of counting system has significant difference between RIA(CV=2. 4%') and TRFIA(CV = 0. 8%) (F<0. 001). The systematic precision has significant difference between RIA(CV = 3. 6%) and TRFIA (CV = 1. 3%) (P<0. 001). The results indicate that the precision of sampling,counting system and the systemic precision of TRFIA are all better than that of RIA.