Abstract: The method for meaturing is established by the two-step, which enzyme-linked im-munosorbent assay (ELISA) using polyclonal antibody for coating, one monoclonal for labeling with horseradish peroxidase. The sensitivity is 0. 06 mIU/L and analytical recovery of TSH is (98. 4 -101. 4)%. The inter- and intra-assay CV are lower than 9. 1% and 5. 9% respectively. The average concentration of TSH in sera from healthy human (77) is 1. 96 mIU /L. The correlation coefficient of results with TSH IRMA is 0. 992. The assay is a rapid, sensitive, simple and precise method. It is suitable for clinical and research application.