固相抗体包被管法TSH免疫放射分析

Immunoradiometric Assay for TSH by Use of Monoclonal Antibody Coated Tube Method

摘要: 采用 TSH单克隆抗体包被聚苯乙烯试管, 并对抗体包被条件进行了研究, 建立了 TSH免疫放射分析法。结果表明: 在 2～ 8℃下过夜包被, 抗体浓度为 2.1mg/L时, 制备的包被管用于 TSH免疫放射分析, 结合达到最大;不同浓度下包被, 包被温度和时间对制备的包被管的影响不同。TSH免疫放射分析最小检测限为 0.0 5 m IU/L;回收率为 88.4 %～ 10 0.0 %;批内、批间变异系数分别为7.4 %～ 10.9%和 8.7%～ 10.7%。 4 6例健康人血样测定值为 0.3～ 6.2 m IU/L。

Abstract: TSH immunoradiometric assay(IRMA) is developed by using anti TSH monoclonal antibody coated on polystyrene tubes. Different conditions for antibody coating on the tubes are tested and compared. Under the condition of coating for overnight at 2～8 ℃, the maximum binding of the coated tubes in IRMA is obtained when the concentration of the added antibody is up to 2.1 mg/L. The temperature and time during coating have different effects on the binding of the coated tubes in IRMA at different concentration of antibody. The sensitivity of TSH IRMA is 0.05 mIU/L. The recovery is 88.4%～100.0%. The intra and inter assay CV are 7.4%～10.9% and 8.7%～10.7% respectively. The value for normal samples( n =46) is 0.3 ～ 6.2 mIU/L.