钙调神经磷酸酶B亚基的~(125)I标记及其血脑屏障的通透性

Study on~(125)I Labelling and Blood-brain Barrier Counteracting of Calcineurin B

摘要: 钙调神经磷酸酶与老年性痴呆密切相关, 为了解其 B亚基的血脑屏障的通透性, 采用 Iodogen法对钙调神经磷酸酶 B亚基 (CNB)进行 12 5I标记, 标记率高达 85 %, 产物 12 5I- CNB比活度为 74 0 GBq· g-1,放化纯度为 95.7%。小鼠静脉注射 12 5I- CNB结果显示血脑屏障能够阻止完整的 CNB进入脑, 但它的降解肽段能够透过血脑屏障进入脑组织

Abstract: Calcineurin B(CNB) is labeled with 125 I by iodogen method. The labeling yield is up 85%,the specific activity is 740 GBq·g -1 and the radiochemical purity is 95.7%.The blood brain barrier counteracting of 125 I CNB is investigated in rats' brain. The results show that blood brain barrier can counteract intact CNB into brain, but can not counteract it's decomposed peptide into brain.