H22肝癌细胞与正常小鼠肝细胞胰岛素受体表达的对比

Comparation of the Expression of Insulin Receptor to H22 Hepatoma Cells With Normal Liver Cells of Mice

摘要: 本实验采用氯胺 T法进行了胰岛素的 12 5I标记。通过体外受体饱和结合实验及竞争结合实验观察了 H2 2肝癌细胞与正常小鼠肝细胞胰岛素受体的结合特性, 并进行 Scatchard分析, 计算出两种细胞的最大结合容量 ( Bmax)与平衡解离常数 ( Kd)。H2 2肝癌细胞胰岛素受体 Bmax为每个细胞 5.61± 1.10 amol,Kd 为 1.81± 0.56nmol/L;正常小鼠肝细胞胰岛素受体 Bmax为每个细胞 3.2 2± 0.81amol,Kd 为 2.10± 0.91nmol/L。H2 2肝癌细胞胰岛素受体 Bmax显著高于正常肝细胞 ( P<0.0 5), 而两种细胞胰岛素受体与 12 5I-胰岛素的亲和力无显著性差异 ( P>0.0 5)。

Abstract: Insulin is labeled with 125 I by Ch T method. Receptor binding assay of 125 I insulin to H22 hepatoma cells and normal mice liver cells are performed respectively. Binding data are calculated according to Scatchard analysis using the ligand program.Statistical comparison is made with the paired t test. K d values of H22 hepatoma cells and normal mice liver cells are 1.81± 0.56 nmol/L and 2.10±0.91 nmol/L respectively, values of B max are 5.61±1.10 amol per cell and 3.22±0.81 amol per cell respectively. H22 hepatoma cells have a significantly higher B max than normal mice liver cells( P <0.05), while the two K d values have little difference( P >0.05).