Abstract: The character of targeting human hepatoma cell of mitoxantrone loaded immunonanosphere(HAb18 DHAQ BSA NP) for anti hepatoma in vitro is studied using rosset forming test, their blocking test, immunofluorescent test and its blocking test. The action of targeting killing human hepatoma cell is studied also using 3H TdR incorporation assay. The results show that HAb18 DHAQ BSA NP can target to bind human hepatoma cell effectively specifically, and has the specific action for killing off human hepatoma cells, which is decided by human hepatoma monoclonal antibody HAb18.