~(59)Fe示踪法测定纳米级磁性氧化铁在小鼠体内的分布

Determining the Distribution of Nano-particles Magnetic Iron Oxide in Mice by ~(59)Fe Tracer

摘要: 将 59Fe标记大分子葡聚糖包埋的磁性氧化铁 (SUMR)注入小鼠体内, 测定其在小鼠血液、心脏、肺、肝脏、脾脏、双肾、股骨、肌肉和尿液等组织和脏器中的放射性, 以观察纳米级磁性氧化铁磁共振造影剂在体内的分布状况。结果表明: 放射性标记的磁性氧化铁主要集中在肝和脾, 其聚集量分别于注入后 30 min和 2 4 h达到最高, 分别为 (95.0 7± 8.2 7) % ID/ g和 (53.93± 5.94 ) % ID/ g。血液中的放射性初始下降很快, 然后逐渐回升, 于注药后 15d达到最大, 而后随时间延长再次逐步降低, 至 6 0 d恢复正常。 SUMR有可能用于肝和脾的磁共振造影增强剂

Abstract: To observe the distribution of nano-particles iron oxide as a magnetic resonance contrast agent in mice, magnetic iron oxide(SUMR) is coated with macromolecular labeled by 59 Fe and the distribution in blood, heart, lung, liver, spleen, kidneys, bone, muscle and urine of mice are determined. Main uptake of 59 Fe-SUMR is in liver and spleen. The maximum is 95.07±8.27 %ID/g and 53.93±5.94 %ID/g at 30 min and 24 h after injection of 59 Fe-SUMR, respectively. 59 Fe-SUMR in blood is initially rapid decreased, then gradually increased and the peak value is appeared at 15 days after injection. The normal level of 59 Fe-SUMR is achieved at 60 days after injection. SUMR is promised to well be used as a magnetic resonance contrast agent for liver and spleen diseases.