~(99)Tc~m-抗纤维蛋白单克隆抗体对兔动静脉血栓的放免显像

RADIOIMMUNOIMAGING OF ARTERIAL AND VENOUS THROMBI IN RABBIT MODEL USING 99 Tc m LABELED MONOCLONAL ANTIFIBRIN ANTIBODY

摘要: 以亚氨基噻吩盐酸盐（２ ＩＴ）法修饰抗纤维蛋白的单克隆抗体（ＭｃＡｂ）ＳＺ６３，采用９９Ｔｃｍ标记葡庚糖酸钠（ＧＨ）配体交换法标记。制备实验性兔股动脉和股静脉血栓模型后，经耳缘静脉注入９９Ｔｃｍ  ＳＺ６３进行ＳＰＥＣＴ显像。９９Ｔｃｍ标记非特异性鼠ＩｇＧ作为阴性对照。显像结果表明，兔陈旧血栓显像清晰，其最佳显像时间为注射９９Ｔｃｍ  ＳＺ６３后２～４ｈ；９９Ｔｃｍ标记非特异性ＩｇＧ，未见血栓显像。因此９９Ｔｃｍ  ＳＺ６３具有活体内血栓定位导向能力，显像时间短，用于血栓性疾病的放免显像有一定的可行性。

Abstract: The anti fibrin McAb SZ 63 is labeled by using the method of 2 iminothiolane modification and 99 Tc m glucoheptonate(GH) transchelation. The experimental femoral arterial and venous thrombi are prepared in rabbits, then 99 Tc m SZ 63 is injected into ear edge vein and imaged by SPECT. 99 Tc m labeled murine IgG is used as a negative control. The old arterial and venous thrombi in rabbits are clearly imaged 2 to 4 h after injection of 99 Tc m SZ 63. McAb SZ 63 would be a potential agent for diagnosis and therapy of thrombotic disease.