乳糖化人生长激素的放免分析及其体内动力学的研究

  • 摘要: 用放射免疫分析方法(RIA)分析了鼠肝、肾匀浆中乳糖化人生长激素(hGH-L)和人生长激素(hGH)的含量,探讨了hGH-L的体内动力学行为,并对该分析测定方法进行了方法学评价。结果表明,hGH-L具有良好的体内动力学特征:其肝摄取总量大于hGH、肾摄取总量远小于hGH,且其肝、肾的清除均快于hGH。该分析方法的灵敏度、回收实验、重复性,稀释线性均符合RIA的质量要求。

     

    Abstract: STUDY ON RIA OF LACTOSAMINATED HUMAN GROWTH HORMONE AND ITS INVIVO KINETICS Li Yunchun\ Guan Changtian\ Li Jingqian\ Liu Ying(The First Hospital of West China University of Medical Science s, Chengdu 610041)Chen Zhelian\ Li Tongling\ Pang Qijie(Medicine Institute, West China University of Medical Sciences, Chengdu 610041)Abstract The contents of lactosaminated human growth horm one (hGHL) and human growth houmone (hGH) in livers and kidneys of mice are de termined, and evaluated on the RIA. The results demonstrate that the total amoun t of uptake of hGHL is more than hGH in liver, and less than hGH in kidney, an d the remove is faster than hGH in liver and kidney. The sensitivity, recovery o f experiment, repetition character and dilution linear of the method accord with the demands of RIA quality.

     

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