白细胞介素-8的~(125)I标记

  • 摘要: 用Bolton-Hunter(HPNS)试剂作为联结剂,制备125I-白介素-8(IL-8)。首先用125I-NaI标记HPNS,标记率为(57.7±18.9)%,然后以pH8.5的硼酸缓冲液为标记介质,用125I-HPNS联接IL-8。分离纯化后125I-IL-8的放化纯度为90.2%±4.5%。

     

    Abstract: STUDY ON INTERLEUKIN8 LABELED WITH 125IFan Wo\ Qian Jianhua\ Zhang Yi\ Shen Weiming\ Zhu Benxing(Department of Radiomedicine, Suzhou Medical College, 215007)ABSTRACTInterleukin8 is labeled with 125I by connection method with BoltonHunter agent(HPNS). The labeling yield and the radiochemical purity of 125I in the labeling to HPNS are 57.7%±18.9% and 62.4%±7.5%, respectively. The radiochemical purity 90.3%±4.4% of 125IIL8 is obtained and not changed during 15 days in the medium of protective protein. The labeling method is applicable but not ideal.Key words 125I labeling\ interleukin8\ connection method with BoltonHunter agentradiochemical purity labeling yield

     

/

返回文章
返回