甲胎蛋白干血滤纸免疫放射分析试剂盒的研制

摘要: 用氯胺Ｔ法制备１２５Ｉ－ＡＦＰＭｃＡｂ，用ＡＦＰＭｃＡｂ包被试管，以全血作标准，建立了ＡＦＰ干血滤纸片免疫放射分析方法。该方法灵敏度为７．２μｇ／Ｌ，批内变异系数为５．１％—１０．０％，批间变异系数为４．７％—８．６％，正常参考值≤１０μｇ／Ｌ，与血清ＡＦＰ免放分析方法比较，相关系数ｒ＝０．９７９

Abstract: DEVELOPMENT OF AFP BLOOD SPOTIMMUNORADIOMETRIC ASSAYChen Jian\ Liu Yibing\ Han Shiquan\ Wang Yanzhen(Depantment of Isotope, China Institute of Atomic Energy, Beijing 102413)ABSTRACTTwo AFP McAb are used in AFP blood spot immunoradiometric assay. One is labeled with iodine125 and the other is coated to the test tubes. The whole blood standards are spotted on filter paper. The measurement range is 10-800 μg/L. The sensitivity is no more than 7.2 μg/L. The intra and interassay coefficient of variation are 5.1%-10.0% and 4.7%-8.6% respectively. The normal value is less than 10 μg/L. The corretation coefficient comparing with the IRMA of serum AFP is 0.979.Key words alphafectoprotein blood spot immunoradiometric assay McAb