~(131)I 后标记法检测 DNA-蛋白交联物

摘要: 应用１３１Ｉ后标记方法，在整体动物水平，检测因环境污染物和化学致癌物而产生的ＤＮＡ－蛋白交联物（ＤＰＣｓ）。研究结果表明，建立最佳１３１Ｉ后标记法反应条件，能有效、灵敏、快速和简便地检测出由食品中常见的化学致癌物黄曲霉毒素Ｂ１（ＡＦＢ１）和亚硝胺引起的大鼠肝脏ＤＰＣｓ的形成量，为该法广泛性应用于检测ＤＰＣｓ展示了良好的前景。

Abstract: DETECTION OF DNAPROTEIN CROSSLINKS BY 131I POSTLABELING METHODYan Ping Liao Huizhen① Zhu Pingping①(Department of Nuclear Medicine,Fujian Medical University,Fuzhou 350004)(①Nutritive Hygiene,Fujian Medical University,Fuzhou 350004) ABSTRACT131I postlabeling method is developed to detect DNAprotein crosslinks(DPCs) induced by environmental contaminants and carcinogens in the level of whole animal. The results demonstrate that mouse liver DPCs caused by carcinogens—aflatoxin B1(AFB1) and nitrosamine in food can be examined simply,effectively,rapidly and sensitively using the established optiumum reaction conditions of 131I postlabeling method. This reveals favorable prospects of 131I postlabeling method to detect widely DPCs.Key words 131I postlabeling DNAprotein crosslinks(DPCs) aflatoxin B1(AFB1) nitrosamine