Abstract: STUDY OF THE FEASIBILITY OF ANTIBODY PURIFICATION IN ACIDIC ISOFERRITIN RADIOIOIMMUNOASSSAYLi Wenxin;Wang Guilian;Huang Biao;Zhang Lianfen;Tao Yonghui;Jin Jian(Jiangsu Institute of Nuclear Medicine,Wuxi 214063)Abstract:The high purity acidic isoferritin (AIF) extracted from the human heart tissue with a medifiedtechnology is coupled to cyanogen bromide-activated Sepharoce CL-6B to preP8re AIF-Sepharose CL6B affinity chromatiographic column. The rabbit anti-AIF serum purified from this column is used toestablish a new AIF RIA. The data obtained are processed by the 4-PL method. T'he intra- and interCV are 1. 65% and 9. 71 %, respectively. The sensitivity, ED50, recovery rate and effective assayrange are 1. 89μg/l, 27. 50 μg/l, 102% and 7. 0-369. 6μg/l, respetively. No cross-reaction withAFP、CEA and LF is oberved and the cross-reaction with ferritin is only 1. 74 %. The serum AIF levels in 30 male or female normal controls are 16. 76±6. 98μg/l and 13. 32±6. 25μg/l, respectively. No statistical difference is observed(P＞0. 05). But the serum AIF levels in 10 patients ofhepotocarcinoma are 36. 09±15. 84 μg/l. being strikingly higher than those of normal controls (P＜0. 001 ). This suggests that the method of purifying the rabbit anti-AIF serum by affinity chromatography is fasiible and will help to improve the sensitivity, spectificity, accuracy and clinical effectivenessof the AIF RIA.Keywords: acidic isoferritin;radioimmunoassay; affinity chromatography cross-reactionhepatocarcinoma