利用F蛋白的放射免疫分析判断肝损伤程度

  • 摘要: F蛋白是肝细胞浆中特有的标志物,从新鲜人肝中分离纯化F蛋白,制备兔抗人肝F蛋白血清和125I-F蛋白,采用聚乙二醇复合兔血清球蛋白分离试剂建立起的F蛋白放射免疫分析方法,检测范围5-400ng/ml(血清)。正常人血清F蛋白含量186±31ng/ml,肝F蛋白含量为202±50μg/g,急性乙型肝炎患者血清F蛋白含量显著高于正常范围。检出率达89.6%。

     

    Abstract: DIANOSINGLIVERDAMAGEBYRIAOFFPROTEINLiHuaifenNiuHuishengZhangFuhua(InstituteofRadiationMedicine,ChineseAcademyofMedicalSciences,Tianjin300192)ABSTRACTFproteinisa44kDacytoplasmicproteinofunknownfunctionwhichisfoundmostlyintheliverandcirculatesatmuchlowerconcentrationintheserum.SerumFproteinhasbeenmeasuredusingasensitiveradioimmunoassay.Theproteinconcentrationinserafrom36healthyhumansis186±31ng/mlandareabnormalyhigherfor52outof58patients(89.6%)withhepatocelulardiseases.Thisnewmarkermaybeofvalueinthediagnosisandtreatmentofliverdamage.KeywordsFproteinRIAliverdamage

     

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