Abstract:
125I┐LABELLINGOFMULTIPLEPEPTIDEHORMONESMan-yinW.TsoL.C.Fu(RadioisotopeUnitoftheUniversityofHongKong)K.L.Yu(ZoologyDepartmentoftheUniversityofHongKong)ABSTRACTFourpeptidehormones,namelymammaliangonadotropinreleasinghormone(GnRH)prohormonefragment(5-16),chickenGnRH-Ⅱ,salmonGnRHandneuropeptideYareconcurrentlyradiolabeledwith125Iinasingleiodinationreaction.Iodinatedpeptidesaresepa-ratedbyreversephaseliquidchromatographyusingtheSEP-PAKC18cartridgewithstepwisegradientsofacetonitrile/trifluoroaceticacidasmobilephase.Thequantitativeyieldandqual-ityoftheiodinatedpeptidesgeneratedinthismultiplepeptidesiodinationprocedurearetest-edwithrespecttotheirapplicationasatracerinspecificradioimmunoassays.Theresultsshowthatthetotalyieldofradiolabeling,thespecificactivityandtheimmunospecificityofthe125I-peptidesgeneratedinthisprocedurearecomparabletothoseoftheradiolabeledpep-tidesobtainedbyseparateiodinationreactions.Keywordsmultiplepeptideiodinationneuropeptidesgonadotropinreleasinghor-mone