神经肽类激素的混合碘标记方法

  • 摘要: 介绍一种快速的混合碘标记肽类激素的方法,供放射免疫测定研究鱼类神经肽激素的生殖生理过程。四种神经肽激素可同时被125I标记,选用不同的极性溶液逐一从一种反相极性色层柱(SEP-PAKC18)上分离。利用这种混合碘标记法,不论在肽的产率、比活度或免疫活性方面,均不亚于用单个肽标记法取得的结果。

     

    Abstract: 125I┐LABELLINGOFMULTIPLEPEPTIDEHORMONESMan-yinW.TsoL.C.Fu(RadioisotopeUnitoftheUniversityofHongKong)K.L.Yu(ZoologyDepartmentoftheUniversityofHongKong)ABSTRACTFourpeptidehormones,namelymammaliangonadotropinreleasinghormone(GnRH)prohormonefragment(5-16),chickenGnRH-Ⅱ,salmonGnRHandneuropeptideYareconcurrentlyradiolabeledwith125Iinasingleiodinationreaction.Iodinatedpeptidesaresepa-ratedbyreversephaseliquidchromatographyusingtheSEP-PAKC18cartridgewithstepwisegradientsofacetonitrile/trifluoroaceticacidasmobilephase.Thequantitativeyieldandqual-ityoftheiodinatedpeptidesgeneratedinthismultiplepeptidesiodinationprocedurearetest-edwithrespecttotheirapplicationasatracerinspecificradioimmunoassays.Theresultsshowthatthetotalyieldofradiolabeling,thespecificactivityandtheimmunospecificityofthe125I-peptidesgeneratedinthisprocedurearecomparabletothoseoftheradiolabeledpep-tidesobtainedbyseparateiodinationreactions.Keywordsmultiplepeptideiodinationneuropeptidesgonadotropinreleasinghor-mone

     

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