Abstract: EVALUATION OF THE APPLICATION FOR ~(125)I-TESTOSTERONE RADIOIMMUNOASSAY BY SOLID PHASE SEPARATION Tang Tao（Laboratory of Endocrinology,Anhui Provincial, Hefei 230001）ABSTRACT ~(125)I-serum testcoterone radioimmunoassay by the solid phase separation is evaluated. Themethod＇s sensitivity is 0．15 mol／l．The intro- assay CV is about 5．7％and the inter- assay CV isabout 15．1％. The immune reaction is steady basically in 2-3h．The B／T value（x±SD）is 54．43％±3．51％，50．63％±4．35％and 53．23％±2．21％respectively for the reaction time of120，15 0 and 1 8 0 min. The RIA determination of serum or plasma testorterene for male＇s and　female＇s sex disease out-patients is performed with both solid phase separation and liquid scintillationtechnique at the same time. Comparing with each relevent normal range，the results indicate that thecoincident rate is 94．1％. Key words ~(125)I-testosterone solid phase separation radioimmunoassay