Abstract: PREPARATION OF ~(186)Re（Sn）-HEDP AND THE COMPARATIVE ANALYSES OF CHROMATOGRAPHICAL METHODS Wang Fan Jin Xiaohai Liu Yuemin Du JinZhao Xiuyan Chen Daming Bai Suzhen（Department of Radioisotope, China Institute of Atomic Energy, Beijing 102413）ABSTRACTThe labeling technology of ~(186)Re（Sn）-HEDP is described, and a complex column（in SephadexG-75 and 717# resin）chromatographic analysis method is studied. Meanwhile, the comparative analyses, on the UV absorption and radioactive determination of purified ~(186)Re（Sn）-HEDP and unpurified~(186)Re（Sn）－HEDP are carried out by HPLC. The relative HPLC spectrums to be obtained are basicallycorrespondent with the spectrums of ~(99)Tc~m-HEDP、 ~(186)Re（Sn）-HEDP quoted from reference, and contamination contents of our product are also less than that one. Experimental results demonstrate thatdouble-paper stripe chromatographic system are unapplicable to analysis radiochemical purity of labeledcompounds especially for the situations of unsufficient reduction of ~(186)ReO_4 and low labeling rate. Thecomplex column chromatographic system used for the determination of radiochemical purity is both simple and rapid. Animal experiments in rats indicate that the radioactive absorption of thyroid and liverfor purified ~(186)Re （Sn）-HEDP is obviously less than unpurified one.Key words bone tumor Rhenium-186 HEDP chromatapraphical analysis HPLC