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Tamm-Horsfall糖蛋白放免分析测定盒的研制

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作者. Tamm-Horsfall糖蛋白放免分析测定盒的研制[J]. 同位素, 1992, 5(2): 88-88. DOI: 10.7538/tws.1992.05.02.0088
引用本文: 作者. Tamm-Horsfall糖蛋白放免分析测定盒的研制[J]. 同位素, 1992, 5(2): 88-88. DOI: 10.7538/tws.1992.05.02.0088
A RADIOIMMUNOASSAY KIT FOR TAMM-HORSFALL GLYCOPROTEIN[J]. Journal of Isotopes, 1992, 5(2): 88-88. DOI: 10.7538/tws.1992.05.02.0088
Citation: A RADIOIMMUNOASSAY KIT FOR TAMM-HORSFALL GLYCOPROTEIN[J]. Journal of Isotopes, 1992, 5(2): 88-88. DOI: 10.7538/tws.1992.05.02.0088

Tamm-Horsfall糖蛋白放免分析测定盒的研制

A RADIOIMMUNOASSAY KIT FOR TAMM-HORSFALL GLYCOPROTEIN

  • 摘要: 采用氯化钠沉淀及SephadexG-100层析方法,从正常人尿中提纯了Tamm-Horsfall糖蛋白(THP)。用提纯的THP免疫家免制备了THP抗血清,抗体工作液滴度1:20000至1:40 000,亲和常数0.99×10~(10)l·mol~1,与人β_2-mG、白蛋白和IgG交叉反应分别小于0.02%,0.0001%和0.0004%。并采用双抗加PEG的方法制备了THP放免分析测定盒,对人血清和尿中THP进行测定。测定范围50—2000ng/ml,批内及批间变异系数分别为(1.6-2.9)%(n=6)和(2.3-4.2)%(n=6),回收率(96.2—103.9)%,正常人血清THP为(123.3±21.9)ng/ml(n=14),24小时尿THP排泄量为(29.0-43.9)mg(n=3)。
    Abstract: Tamm-Horsfall glycoprotein (THP) was prepared and purefied from urine by salt precipitation and chromatography on Sephadex G-100. An antiserum was raised by immunizing rabbits. The antiserum had a working titer of 1: 20 000 to 1: 40 000 and the affinity constant was 0.99×10~(10)l. mol~(-1). The cross reaction with β_2-MG, HSA and IgG was lower than 0.02%, 0.000 1% and 0.000 4% respectively. A RIA kit was developed for determination of THP in human serum and urine. The standard range was 50—200 0 ng/ml and recoveries varied from 96.2% to 103.9%. Intra-and Inter-assay coefficient of variation were (1.6—2.9)% and (2.3—4.2)% respectively. The normal range of THP was 123. 3±21.9 ng/ml(X±SD, n=14) in serum, and (29.0—43.9) mg/ 24 h (n=3)in urine.
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    [3] Hoyer, J. R. et al., Kidney Int., 16, 277 (1979).
    [4] Wirdnam, P. K. and Milner, RDG, Nephron, 40, 362 (1985).
    [5] 姚建等,中华肾脏病杂志,4(1), 2(1988) 。
    [6] 朱菊红等,生物化学与生物物理学报,20(2), 105(1988) 。
    [7] 中国原子能科学研究院,THP放免测定盒说明书。
    [8] Dawney, A, et al. Biochem. J., 206, 461(1982).
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出版历程
  • 收稿日期:  1991-07-09
  • 刊出日期:  1992-05-19

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