抗3,3′,5′-三碘甲腺原氨酸(反T_3,rT_3)抗血清的制备

PREPARATION AND CHARACTERIZATION OF 3,3′,5′-TRIIODOTHYRONINE(rT_3) ANTISERUM

摘要: <正> 一、前言放射免疫分析测定人血清中rT_3含量,在甲状腺激素的代谢及甲状腺生理病理的研究和临床检验方面都有重要的意义。但血清中rT_3的含量较低,并且rT_3的类似物T_4,T_3的含量较高,这就需要在rT_3的放射免疫分析中有一个特异性好的、亲和力高的抗血清。我们以BSA-rT_3甲酯为免疫原,免疫家兔制备了rT_3抗血清,满足了rT_3放射免疫分析的需要。

Abstract: An antiserum against rT_3 was obtained by immunizing rabbit with a conjugate of rT_3 methylester and bovine serum albumin The antiserum had a final dilution of 1: 15000 to 1: 42000, and the affinity constant was 1. 23×10~(10) to 10.5 × 10~(10) mol~(-1) l, The cross reaction with T_4, T_3 showed lower than 0.02% and 0.032% respectively.