Abstract: In this paper, a method for synthesis of ~(14)C-dehydrocorydaline chloride is described. In the presence of sodium hydroxide, acetonylpalmatine is reacted with ~(14)C-methyl iodide in sealed glass ampoule to give ~(14)C-13-methylpalmatine iodide which is then coverted to chloride. The radiochemical purity of ~(14)C-dehydrocorydaline determined by TLC is over 98％ and the labelling efficiency is 540％.